Regulatory

Part:BBa_K414007:Design

Designed by: Christian Copley   Group: iGEM10_Nevada   (2010-10-19)

DREB1C promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

DREB1C was derived from the region 500 bp up stream of the DREB1C start codon. The first 27 base pairs were omitted due to an Xba1 site in this region. Extensive research shows no expression relevance to this region. (Shinozaki, 1998)

Source

Arabidopsis Genome PCR product

References

Zabta K. Shinwari, Kazuo Nakashima, Setsuko Miura, Mie Kasuga, Motoaki Seki, Kazuko Yamaguchi-Shinozaki, and Kazuo Shinozaki, An Arabidopsis Gene Family Encoding DRE/CRT Binding Proteins Involved in Low-Temperature-Responsive Gene Expression, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 250, 161–170 (1998)</p>

Colleen J. Doherty, Heather A. Van Buskirk, Susan J. Myers, and Michael F. Thomashowa, Roles for Arabidopsis CAMTA Transcription Factors in Cold-Regulated Gene Expression and Freezing Tolerance, The Plant Cell, Vol. 21: 972–984, March 2009

Kazuo Nakashima and Kazuko Yamaguchi-Shinozaki, Regulons involved in osmotic stress-responsive and cold stress-responsive gene expression in plants, Physiologia Plantarum 126: 62–71. 2006

Satoshi Kidokoro, Kyonoshin Maruyama, Kazuo Nakashima, Yoshiyuki Imura2, Yoshihiro Narusaka, Zabta K. Shinwari, Yuriko Osakabe, Yasunari Fujita, Junya Mizoi, Kazuo Shinozaki, and Kazuko Yamaguchi-Shinozaki, The Phytochrome-Interacting Factor PIF7 Negatively Regulates DREB1 Expression under Circadian Control in Arabidopsis, Plant Physiol. Vol. 151, 2009